6FB0
Crystal Structure of a Tailored I-CreI Homing Endonuclease Protein (3115 variant) in complex with its target DNA (Haemoglobin beta subunit gene) in the presence of Calcium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-03-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.87 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.437, 67.673, 83.525 |
| Unit cell angles | 90.00, 96.75, 90.00 |
Refinement procedure
| Resolution | 33.837 - 2.150 |
| R-factor | 0.1799 |
| Rwork | 0.176 |
| R-free | 0.22940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g9y |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.154 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6_289) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.810 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.070 | 0.460 |
| Number of reflections | 26697 | |
| <I/σ(I)> | 10.5 | |
| Completeness [%] | 100.0 | |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M Calcium Acetate 0.1M Sodium Acetate pH=4.6 35% 1,2-propanediol |
