6FB0
Crystal Structure of a Tailored I-CreI Homing Endonuclease Protein (3115 variant) in complex with its target DNA (Haemoglobin beta subunit gene) in the presence of Calcium
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA endonuclease I-CreI | polymer | 153 | 17528.2 | 1 | Pfam (PF00961) UniProt (by SIFTS) (P05725) | Chlamydomonas reinhardtii (Chlamydomonas smithii) | 23S rRNA intron protein |
| 2 | B (B) | DNA endonuclease I-CreI | polymer | 154 | 17583.2 | 1 | Pfam (PF00961) UniProt (by SIFTS) (P05725) | Chlamydomonas reinhardtii (Chlamydomonas smithii) | 23S rRNA intron protein |
| 3 | C (D) | DNA (5'-D(*TP*CP*AP*GP*AP*CP*TP*TP*CP*TP*CP*CP*AP*CP*AP*GP*GP*AP*GP*TP*CP*AP*GP*A)-3') | polymer | 24 | 7338.8 | 1 | synthetic construct | ||
| 4 | D (F) | DNA (5'-D(*TP*CP*TP*GP*AP*CP*TP*CP*CP*TP*GP*TP*GP*GP*AP*GP*AP*AP*GP*TP*CP*TP*GP*A)-3') | polymer | 24 | 7400.8 | 1 | synthetic construct | ||
| 5 | E, F, J, K (A, B) | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
| 6 | G, L, M, N, O (A, B) | ACETATE ION | non-polymer | 59.0 | 5 | Chemie (ACT) | |||
| 7 | H, I (A) | S-1,2-PROPANEDIOL | non-polymer | 76.1 | 2 | Chemie (PGO) | |||
| 8 | P, Q, R, S (A, B, D, F) | water | water | 18.0 | 164 | Chemie (HOH) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 49850.9 | |
| Non-Polymers* | Number of molecules | 11 |
| Total formula weight | 607.7 | |
| All* | Total formula weight | 50458.6 |
