6F60

The x-ray structure of bovine pancreatic ribonuclease in complex with a five-coordinate platinum(II) compound containing a sugar ligand

Summary for 6F60

• Entry DOI: 10.2210/pdb6f60/pdb
• Descriptor: Ribonuclease pancreatic, SULFATE ION, five-coordinate platinum(II) compound, ... (5 entities in total)
• Functional Keywords: ribonuclease, platinum(ii) compound, five-coordinate, sugar ligand, cytotoxicity, hydrolase
• Biological source: Bos taurus (Bovine)
• Cellular location: Secreted: P61823
• Total number of polymer chains: 2
• Total formula weight: 28683.76

Authors

Merlino, A.,Ferraro, G. (deposition date: 2017-12-04, release date: 2018-04-25, Last modification date: 2024-11-20)

Primary citation

Cucciolito, M.E.,D'Amora, A.,De Feo, G.,Ferraro, G.,Giorgio, A.,Petruk, G.,Monti, D.M.,Merlino, A.,Ruffo, F.
Five-Coordinate Platinum(II) Compounds Containing Sugar Ligands: Synthesis, Characterization, Cytotoxic Activity, and Interaction with Biological Macromolecules.
Inorg Chem, 57:3133-3143, 2018

PubMed Abstract: This article describes the synthesis and characterization of novel cationic five-coordinate Pt(II) complexes containing nitrogen sugar-based ligands. The cytotoxicity of the complexes was evaluated on different cell lines with the expectation that both the coordinative saturation and the sugar moiety cooperate to enhance their biological activity. In fact, the complexes resulted to be more active than cisplatin but still with little selectivity. They activate the apoptosis pathway. Binding of representative compounds with DNA was studied by ethidium bromide displacement assay and circular dichroism. Binding to model proteins was also investigated; the X-ray structure of the adduct formed in the reaction between a representative compound and the model protein bovine pancreatic ribonuclease was obtained. The structure discloses an unprecedented interaction between a five-coordinate Pt(II) moiety and a His side chain.

PubMed: 29509011
DOI: 10.1021/acs.inorgchem.7b03118

Experimental method

X-RAY DIFFRACTION (1.14 Å)