6F60
The x-ray structure of bovine pancreatic ribonuclease in complex with a five-coordinate platinum(II) compound containing a sugar ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-08 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.780, 32.519, 72.337 |
| Unit cell angles | 90.00, 90.19, 90.00 |
Refinement procedure
| Resolution | 72.340 - 1.140 |
| R-factor | 0.14123 |
| Rwork | 0.138 |
| R-free | 0.19440 |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.534 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.340 | 1.180 |
| High resolution limit [Å] | 1.140 | 1.140 |
| Rmerge | 0.045 | |
| Rpim | 0.034 | 0.805 |
| Number of reflections | 80973 | |
| <I/σ(I)> | 8.7 | 0.9 |
| Completeness [%] | 95.5 | 92.5 |
| Redundancy | 2.4 | 2.3 |
| CC(1/2) | 0.998 | 0.363 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 22% PEG 4K 0.01 M sodium citrate pH 5.1 |
