6F09

Binary complex of 14-3-3 zeta with ubiquitin specific protease 8 (USP8) pSer718 peptide

6F09 の概要

• エントリーDOI: 10.2210/pdb6f09/pdb
• 分子名称: 14-3-3 protein zeta/delta, Ubiquitin carboxyl-terminal hydrolase 8 (3 entities in total)
• 機能のキーワード: 14-3-3, usp8, phosphosite, binary complex protein-peptide, signaling protein
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Cytoplasm : P63104 P40818
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 111689.88

構造登録者

Centorrino, F.,Ballone, A.,Ottmann, C.,Guo, S.,Leysen, S. (登録日: 2017-11-17, 公開日: 2018-03-07, 最終更新日: 2024-10-16)

主引用文献

Centorrino, F.,Ballone, A.,Wolter, M.,Ottmann, C.
Biophysical and structural insight into the USP8/14-3-3 interaction.
FEBS Lett., 592:1211-1220, 2018

PubMed Abstract: The ubiquitin-specific protease 8 (USP8)/14-3-3 protein-protein interaction has recently been shown to exert a significant role in the pathogenesis of Cushing's disease (CD). USP8 is a deubiquitinase that prevents epidermal growth factor receptor (EGFR) degradation. Impairment of 14-3-3 binding leads to a higher deubiquitination of EGFR and results in a higher EGFR signaling and an increased production of adrenocorticotropic hormone. Here we report the high-resolution crystal structure of the 14-3-3 binding motif of USP8 surrounding Ser718 in complex with 14-3-3ζ and characterize the interaction with fluorescence polarization and isothermal titration calorimetry. Furthermore, we analyze the effect of USP8 mutations identified in CD on binding to 14-3-3.

PubMed: 29473952
DOI: 10.1002/1873-3468.13017

実験手法

X-RAY DIFFRACTION (1.594 Å)