6EVK

Crystal structure of bat influenza A/H17N10 polymerase with viral RNA promoter and cap analogue m7GTP

6EVK の概要

• エントリーDOI: 10.2210/pdb6evk/pdb
• 分子名称: Polymerase acidic protein, RNA-directed RNA polymerase catalytic subunit, Polymerase basic protein 2, ... (9 entities in total)
• 機能のキーワード: influenza virus, rna-dependent rna polymerase, cap analogue, viral protein
• 由来する生物種: Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)); 詳細
• 細胞内の位置: Host nucleus : H6QM90
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 277030.17

構造登録者

Pflug, A.,Cusack, S. (登録日: 2017-11-01, 公開日: 2017-12-13, 最終更新日: 2024-01-17)

主引用文献

Pflug, A.,Gaudon, S.,Resa-Infante, P.,Lethier, M.,Reich, S.,Schulze, W.M.,Cusack, S.
Capped RNA primer binding to influenza polymerase and implications for the mechanism of cap-binding inhibitors.
Nucleic Acids Res., 46:956-971, 2018

PubMed Abstract: Influenza polymerase uses short capped primers snatched from nascent Pol II transcripts to initiate transcription of viral mRNAs. Here we describe crystal structures of influenza A and B polymerase bound to a capped primer in a configuration consistent with transcription initiation ('priming state') and show by functional assays that conserved residues from both the PB2 midlink and cap-binding domains are important for positioning the capped RNA. In particular, mutation of PB2 Arg264, which interacts with the triphosphate linkage in the cap, significantly and specifically decreases cap-dependent transcription. We also compare the configuration of the midlink and cap-binding domains in the priming state with their very different relative arrangement (called the 'apo' state) in structures where the potent cap-binding inhibitor VX-787, or a close analogue, is bound. In the 'apo' state the inhibitor makes additional interactions to the midlink domain that increases its affinity beyond that to the cap-binding domain alone. The comparison suggests that the mechanism of resistance of certain mutations that allow virus to escape from VX-787, notably PB2 N510T, can only be rationalized if VX-787 has a dual mode of action, direct inhibition of capped RNA binding as well as stabilization of the transcriptionally inactive 'apo' state.

PubMed: 29202182
DOI: 10.1093/nar/gkx1210

実験手法

X-RAY DIFFRACTION (2.9 Å)