6EG2
Crystal structure of human BRM in complex with compound 16
Summary for 6EG2
| Entry DOI | 10.2210/pdb6eg2/pdb |
| Descriptor | Maltose/maltodextrin-binding periplasmic protein,Probable global transcription activator SNF2L2, N-(5-amino-2-chloropyridin-4-yl)-N'-(4-bromo-3-{[3-(hydroxymethyl)phenyl]ethynyl}-1,2-thiazol-5-yl)urea, ISOPROPYL ALCOHOL, ... (4 entities in total) |
| Functional Keywords | helicase, atpase, chromatin remodeling, inhibitor, mbp fusion, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor |
| Biological source | Escherichia coli O157:H7 More |
| Total number of polymer chains | 1 |
| Total formula weight | 70251.99 |
| Authors | Zhu, X.,Kulathila, R.,Hu, T.,Xie, X. (deposition date: 2018-08-17, release date: 2018-10-31, Last modification date: 2023-10-11) |
| Primary citation | Papillon, J.P.N.,Nakajima, K.,Adair, C.D.,Hempel, J.,Jouk, A.O.,Karki, R.G.,Mathieu, S.,Mobitz, H.,Ntaganda, R.,Smith, T.,Visser, M.,Hill, S.E.,Hurtado, F.K.,Chenail, G.,Bhang, H.C.,Bric, A.,Xiang, K.,Bushold, G.,Gilbert, T.,Vattay, A.,Dooley, J.,Costa, E.A.,Park, I.,Li, A.,Farley, D.,Lounkine, E.,Yue, Q.K.,Xie, X.,Zhu, X.,Kulathila, R.,King, D.,Hu, T.,Vulic, K.,Cantwell, J.,Luu, C.,Jagani, Z. Discovery of Orally Active Inhibitors of Brahma Homolog (BRM)/SMARCA2 ATPase Activity for the Treatment of Brahma Related Gene 1 (BRG1)/SMARCA4-Mutant Cancers. J. Med. Chem., 61:10155-10172, 2018 Cited by PubMed Abstract: SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin subfamily A member 2 (SMARCA2), also known as Brahma homologue (BRM), is a Snf2-family DNA-dependent ATPase. BRM and its close homologue Brahma-related gene 1 (BRG1), also known as SMARCA4, are mutually exclusive ATPases of the large ATP-dependent SWI/SNF chromatin-remodeling complexes involved in transcriptional regulation of gene expression. No small molecules have been reported that modulate SWI/SNF chromatin-remodeling activity via inhibition of its ATPase activity, an important goal given the well-established dependence of BRG1-deficient cancers on BRM. Here, we describe allosteric dual BRM and BRG1 inhibitors that downregulate BRM-dependent gene expression and show antiproliferative activity in a BRG1-mutant-lung-tumor xenograft model upon oral administration. These compounds represent useful tools for understanding the functions of BRM in BRG1-loss-of-function settings and should enable probing the role of SWI/SNF functions more broadly in different cancer contexts and those of other diseases. PubMed: 30339381DOI: 10.1021/acs.jmedchem.8b01318 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.98 Å) |
Structure validation
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