6E7L

Structure of a dimerized UUCG motif

Summary for 6E7L

• Entry DOI: 10.2210/pdb6e7l/pdb
• Descriptor: RNA (5'-R(*GP*GP*GP*CP*UP*GP*CP*AP*CP*UP*UP*CP*GP*GP*UP*GP*CP*UP*GP*CP*CP*C)-3') (2 entities in total)
• Functional Keywords: uucg tetraloop dimer, rna
• Biological source: synthetic construct
• Total number of polymer chains: 1
• Total formula weight: 7018.18

Authors

Montemayor, E.J.,Butcher, S.E. (deposition date: 2018-07-26, release date: 2019-07-31, Last modification date: 2024-04-03)

Primary citation

Montemayor, E.J.,Virta, J.M.,Hagler, L.D.,Zimmerman, S.C.,Butcher, S.E.
Structure of an RNA helix with pyrimidine mismatches and cross-strand stacking.
Acta Crystallogr.,Sect.F, 75:652-656, 2019

PubMed Abstract: The structure of a 22-base-pair RNA helix with mismatched pyrimidine base pairs is reported. The helix contains two symmetry-related CUG sequences: a triplet-repeat motif implicated in myotonic dystrophy type 1. The CUG repeat contains a U-U mismatch sandwiched between Watson-Crick pairs. Additionally, the center of the helix contains a dimerized UUCG motif with tandem pyrimidine (U-C/C-U) mismatches flanked by U-G wobble pairs. This region of the structure is significantly different from previously observed structures that share the same sequence and neighboring base pairs. The tandem pyrimidine mismatches are unusual and display sheared, cross-strand stacking geometries that locally constrict the helical width, a type of stacking previously associated with purines in internal loops. Thus, pyrimidine-rich regions of RNA have a high degree of structural diversity.

PubMed: 31584014
DOI: 10.1107/S2053230X19012172

Experimental method

X-RAY DIFFRACTION (2.59 Å)