6E7L

Structure of a dimerized UUCG motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 41 21 2
Unit cell lengths	42.041, 42.041, 77.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.993 - 2.590
R-factor	0.1792
Rwork	0.176
R-free	0.21020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	ideal A-form RNA single stranded
RMSD bond length	0.012
RMSD bond angle	1.940
Data reduction software	XDS (20180409)
Data scaling software	Aimless (0.7.1)
Phasing software	PHASER (2.8.1)
Refinement software	PHENIX (1.13-2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.860	2.710
High resolution limit [Å]	2.590	2.590
Rpim	0.022	0.593
Number of reflections	2473	294
<I/σ(I)>	20	1.3
Completeness [%]	100.0	100
Redundancy	23.7	25.3
CC(1/2)	1.000	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289	20 mM bis-tris pH 6.5 100 mM sodium HEPES pH 7.4 20 % PEG 3,350 20 % glycerol 10 % MPD