6E7B

13-pf 3-start GMPCPP-human alpha1B/beta3 microtubules

Summary for 6E7B

• Entry DOI: 10.2210/pdb6e7b/pdb
• EMDB information: 8997
• Descriptor: Tubulin beta-3 chain, Tubulin alpha-1B chain, MAGNESIUM ION, ... (5 entities in total)
• Functional Keywords: cytoskeleton, microtubules, recombinant human tubulin, tubulin isotype, structural protein
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 2
• Total formula weight: 97567.95

Authors

Ti, S.C.,Alushin, G.M.,Kapoor, T.M. (deposition date: 2018-07-25, release date: 2018-10-10, Last modification date: 2024-03-13)

Primary citation

Ti, S.C.,Alushin, G.M.,Kapoor, T.M.
Human beta-Tubulin Isotypes Can Regulate Microtubule Protofilament Number and Stability.
Dev. Cell, 47:175-, 2018

PubMed Abstract: Cell biological studies have shown that protofilament number, a fundamental feature of microtubules, can correlate with the expression of different tubulin isotypes. However, it is not known if tubulin isotypes directly control this basic microtubule property. Here, we report high-resolution cryo-EM reconstructions (3.5-3.65 Å) of purified human α1B/β3 and α1B/β2B microtubules and find that the β-tubulin isotype can determine protofilament number. Comparisons of atomic models of 13- and 14-protofilament microtubules reveal how tubulin subunit plasticity, manifested in "accordion-like" distributed structural changes, can accommodate distinct lattice organizations. Furthermore, compared to α1B/β3 microtubules, α1B/β2B filaments are more stable to passive disassembly and against depolymerization by MCAK or chTOG, microtubule-associated proteins with distinct mechanisms of action. Mixing tubulin isotypes in different proportions results in microtubules with protofilament numbers and stabilities intermediate to those of isotypically pure filaments. Together, our findings indicate that microtubule protofilament number and stability can be controlled through β-tubulin isotype composition.

PubMed: 30245156
DOI: 10.1016/j.devcel.2018.08.014

Experimental method

ELECTRON MICROSCOPY (3.5 Å)