6E13

Pseudomonas putida PqqB with a non-physiological zinc at the active site binds the substrate mimic, 5-cysteinyl-3,4-dihydroxyphenylalanine (5-Cys-DOPA), non-specifically but supports the proposed function of the enzyme in pyrroloquinoline quinone biosynthesis.

Summary for 6E13

Entry DOI	10.2210/pdb6e13/pdb
Descriptor	Coenzyme PQQ synthesis protein B, ZINC ION, CHLORIDE ION, ... (5 entities in total)
Functional Keywords	iron-dependent, hydroxylase, metallo beta-lactamase, oxidoreductase
Biological source	Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Total number of polymer chains	1
Total formula weight	34482.14
Authors	Evans III, R.L.,Wilmot, C.M. (deposition date: 2018-07-09, release date: 2019-05-22, Last modification date: 2024-03-13)
Primary citation	Koehn, E.M.,Latham, J.A.,Armand, T.,Evans 3rd, R.L.,Tu, X.,Wilmot, C.M.,Iavarone, A.T.,Klinman, J.P. Discovery of Hydroxylase Activity for PqqB Provides a Missing Link in the Pyrroloquinoline Quinone Biosynthetic Pathway. J.Am.Chem.Soc., 141:4398-4405, 2019 Cited by PubMed: 30811189 DOI: 10.1021/jacs.8b13453 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.349 Å)

Structure validation