6DZL
Ebola virus Makona variant GP (mucin-deleted) in complex with pan-ebolavirus human antibody ADI-15878 Fab
Summary for 6DZL
| Entry DOI | 10.2210/pdb6dzl/pdb |
| EMDB information | 8935 |
| Descriptor | Ebola virus Makona GP1, Ebola virus Makona GP2, Fv domain of ADI-15878 Fab light chain, ... (7 entities in total) |
| Functional Keywords | ebola, antibody, pan-ebolavirus, internal fusion loop, hr1, glycoprotein, viral protein |
| Biological source | Zaire ebolavirus (ZEBOV) More |
| Total number of polymer chains | 12 |
| Total formula weight | 240891.30 |
| Authors | Murin, C.D.,Ward, A.B. (deposition date: 2018-07-05, release date: 2018-09-12, Last modification date: 2024-11-20) |
| Primary citation | Murin, C.D.,Bruhn, J.F.,Bornholdt, Z.A.,Copps, J.,Stanfield, R.,Ward, A.B. Structural Basis of Pan-Ebolavirus Neutralization by an Antibody Targeting the Glycoprotein Fusion Loop. Cell Rep, 24:2723-2732.e4, 2018 Cited by PubMed Abstract: Monoclonal antibodies (mAbs) with pan-ebolavirus cross-reactivity are highly desirable, but development of such mAbs is limited by a lack of a molecular understanding of cross-reactive epitopes. The antibody ADI-15878 was previously identified from a human survivor of Ebola virus Makona variant (EBOV/Mak) infection. This mAb demonstrated potent neutralizing activity against all known ebolaviruses and provided protection in rodent and ferret models against three ebolavirus species. Here, we describe the unliganded crystal structure of ADI-15878 as well as the cryo-EM structures of ADI-15878 in complex with the EBOV/Mak and Bundibugyo virus (BDBV) glycoproteins (GPs). ADI-15878 binds through an induced-fit mechanism by targeting highly conserved residues in the internal fusion loop (IFL), bridging across GP protomers via the heptad repeat 1 (HR1) region. Our structures provide a more complete description of the ebolavirus immunogenic landscape, as well as a molecular basis for how rare but potent antibodies target conserved filoviral fusion machinery. PubMed: 30184505DOI: 10.1016/j.celrep.2018.08.009 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (4.14 Å) |
Structure validation
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