6DS1

Crystal structure of Cj0485 dehydrogenase in complex with NADP+

Summary for 6DS1

• Entry DOI: 10.2210/pdb6ds1/pdb
• Descriptor: Putative oxidoreductase, NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, GLYCEROL, ... (5 entities in total)
• Functional Keywords: dehydrogenase, oxidoreductase
• Biological source: Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
• Total number of polymer chains: 4
• Total formula weight: 125434.41

Authors

Pluvinage, B.,Boraston, A.B. (deposition date: 2018-06-13, release date: 2019-07-10, Last modification date: 2023-10-11)

Primary citation

Garber, J.M.,Nothaft, H.,Pluvinage, B.,Stahl, M.,Bian, X.,Porfirio, S.,Enriquez, A.,Butcher, J.,Huang, H.,Glushka, J.,Line, E.,Gerlt, J.A.,Azadi, P.,Stintzi, A.,Boraston, A.B.,Szymanski, C.M.
The gastrointestinal pathogen Campylobacter jejuni metabolizes sugars with potential help from commensal Bacteroides vulgatus.
Commun Biol, 3:2-2, 2020

PubMed Abstract: Although the gastrointestinal pathogen Campylobacter jejuni was considered asaccharolytic, >50% of sequenced isolates possess an operon for L-fucose utilization. In C. jejuni NCTC11168, this pathway confers L-fucose chemotaxis and competitive colonization advantages in the piglet diarrhea model, but the catabolic steps remain unknown. Here we solved the putative dehydrogenase structure, resembling FabG of Burkholderia multivorans. The C. jejuni enzyme, FucX, reduces L-fucose and D-arabinose in vitro and both sugars are catabolized by fuc-operon encoded enzymes. This enzyme alone confers chemotaxis to both sugars in a non-carbohydrate-utilizing C. jejuni strain. Although C. jejuni lacks fucosidases, the organism exhibits enhanced growth in vitro when co-cultured with Bacteroides vulgatus, suggesting scavenging may occur. Yet, when excess amino acids are available, C. jejuni prefers them to carbohydrates, indicating a metabolic hierarchy exists. Overall this study increases understanding of nutrient metabolism by this pathogen, and identifies interactions with other gut microbes.

PubMed: 31925306
DOI: 10.1038/s42003-019-0727-5

Experimental method

X-RAY DIFFRACTION (2.119 Å)