6DOI

Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid (1.54 Angstrom wavelength): Soak in 0.5 mM EGTA and 200 mM K+ at 21 C

Summary for 6DOI

• Entry DOI: 10.2210/pdb6doi/pdb
• Group deposition: Cleavage of RNA by B. Halodurans Ribonuclease H1 (G_1001001)
• Descriptor: Ribonuclease H, 5'-R(*AP*CP*AP*U)-3' portion of intact RNA (5'-R(*AP*CP*AP*UP*CP*G)-3'), 5'-R(P*CP*G)-3' portion of intact RNA (5'-R(*AP*CP*AP*UP*CP*G)-3'), ... (10 entities in total)
• Functional Keywords: protein-rna-dna complex, double helix, rna hydrolysis, in crystallo catalysis, metal dependent catalysis, monovalent cations, divalent cations, hydrolase-rna-dna complex, hydrolase/rna/dna
• Biological source: Bacillus halodurans C-125; More
• Total number of polymer chains: 4
• Total formula weight: 20185.26

Authors

Samara, N.L.,Yang, W. (deposition date: 2018-06-09, release date: 2018-08-22, Last modification date: 2023-10-11)

• Primary citation: Samara, N.L.,Yang, W.; Cation trafficking propels RNA hydrolysis.; Nat. Struct. Mol. Biol., 25:715-721, 2018; PubMed: 30076410; DOI: 10.1038/s41594-018-0099-4

Experimental method

X-RAY DIFFRACTION (1.948 Å)