6DOI

Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid (1.54 Angstrom wavelength): Soak in 0.5 mM EGTA and 200 mM K+ at 21 C

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Ribonuclease H	polymer	133	15373.3	1	UniProt (Q9KEI9)	Bacillus halodurans C-125	RNase H
2	B (B)	5'-R(*AP*CP*AP*U)-3' portion of intact RNA (5'-R(*AP*CP*AP*UP*CP*G)-3')	polymer	4	1224.8	1		unidentified
3	C (b)	5'-R(P*CP*G)-3' portion of intact RNA (5'-R(*AP*CP*AP*UP*CP*G)-3')	polymer	2	605.4	1		unidentified
4	D (C)	DNA (5'-D(*CP*GP*AP*TP*GP*T)-3')	polymer	6	1824.2	1		unidentified
5	E, F, Q (A, C)	POTASSIUM ION	non-polymer		39.1	3	Chemie (K)
6	G (A)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
7	H, I, J, K (A)	IODIDE ION	non-polymer		126.9	4	Chemie (IOD)
8	L, M, O, P (A)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
9	N, R (A, C)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
10	S, T, U, V (A, B, b, C)	water	water		18.0	164	Chemie (HOH)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	19027.8
Non-Polymers*	Number of molecules	14
	Total formula weight	1157.5
All*	Total formula weight	20185.3

*Water molecules are not included.