6DJW

Crystal Structure of pParkin (REP and RING2 deleted)-pUb-UbcH7 complex

6DJW の概要

• エントリーDOI: 10.2210/pdb6djw/pdb
• 分子名称: RBR-type E3 ubiquitin transferase,RBR-type E3 ubiquitin transferase, Ubiquitin, Ubiquitin-conjugating enzyme E2 L3, ... (4 entities in total)
• 機能のキーワード: ubiquitin, e3 ligase, e2 conjugating enzyme, phosphorylation, mitophagy, parkinson disease, transferase
• 由来する生物種: Bactrocera dorsalis (Oriental fruit fly); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 66676.13

構造登録者

Sauve, V.,Sung, G.,Trempe, J.F.,Gehring, K. (登録日: 2018-05-26, 公開日: 2018-07-04, 最終更新日: 2024-10-30)

主引用文献

Sauve, V.,Sung, G.,Soya, N.,Kozlov, G.,Blaimschein, N.,Miotto, L.S.,Trempe, J.F.,Lukacs, G.L.,Gehring, K.
Mechanism of parkin activation by phosphorylation.
Nat. Struct. Mol. Biol., 25:623-630, 2018

PubMed Abstract: Mutations in the ubiquitin ligase parkin are responsible for a familial form of Parkinson's disease. Parkin and the PINK1 kinase regulate a quality-control system for mitochondria. PINK1 phosphorylates ubiquitin on the outer membrane of damaged mitochondria, thus leading to recruitment and activation of parkin via phosphorylation of its ubiquitin-like (Ubl) domain. Here, we describe the mechanism of parkin activation by phosphorylation. The crystal structure of phosphorylated Bactrocera dorsalis (oriental fruit fly) parkin in complex with phosphorylated ubiquitin and an E2 ubiquitin-conjugating enzyme reveals that the key activating step is movement of the Ubl domain and release of the catalytic RING2 domain. Hydrogen/deuterium exchange and NMR experiments with the various intermediates in the activation pathway confirm and extend the interpretation of the crystal structure to mammalian parkin. Our results rationalize previously unexplained Parkinson's disease mutations and the presence of internal linkers that allow large domain movements in parkin.

PubMed: 29967542
DOI: 10.1038/s41594-018-0088-7

実験手法

X-RAY DIFFRACTION (3.801 Å)