6DFT

Trypanosoma brucei deoxyhypusine synthase

6DFT の概要

• エントリーDOI: 10.2210/pdb6dft/pdb
• 分子名称: Deoxyhypusine synthase, Deoxyhypusine synthase regulatory subunit, SODIUM ION, ... (4 entities in total)
• 機能のキーワード: heterotetramer, rossman fold, pseudoenzyme, nad+, transferase
• 由来する生物種: Trypanosoma brucei; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 533856.78

構造登録者

Tomchick, D.R.,Phillips, M.A.,Afanador, G.A. (登録日: 2018-05-15, 公開日: 2018-08-08, 最終更新日: 2023-10-11)

主引用文献

Afanador, G.A.,Tomchick, D.R.,Phillips, M.A.
Trypanosomatid Deoxyhypusine Synthase Activity Is Dependent on Shared Active-Site Complementation between Pseudoenzyme Paralogs.
Structure, 26:1499-1512.e5, 2018

PubMed Abstract: Trypanosoma brucei is a neglected tropical disease endemic to Africa. The polyamine spermidine is essential for post-translational hypusine modification of eukaryotic initiation factor 5A (eIF5A), which is catalyzed by deoxyhypusine synthase (TbDHS). In trypanosomatids, deoxyhypusine synthase (DHS) activity is dependent on heterotetramer formation between two paralogs, DHSc and DHSp, both with minimal activity on their own due to missing catalytic residues. We determined the X-ray structure of TbDHS showing a single functional shared active site is formed at the DHSc/DHSp heterodimer interface, with deficiencies in one subunit complemented by the other. Each heterodimer contains two NAD binding sites, one housed in the functional catalytic site and the second bound in a remnant dead site that lacks key catalytic residues. Functional analysis of these sites by site-directed mutagenesis identified long-range contributions to the catalytic site from the dead site. Differences between trypanosomatid and human DHS that could be exploited for drug discovery were identified.

PubMed: 30197036
DOI: 10.1016/j.str.2018.07.012

実験手法

X-RAY DIFFRACTION (3.5 Å)