6D2Q

Crystal structure of the FERM domain of zebrafish FARP1

Summary for 6D2Q

• Entry DOI: 10.2210/pdb6d2q/pdb
• Related: 6D21
• Descriptor: FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived) (1 entity in total)
• Functional Keywords: membrane targeting, signaling protein
• Biological source: Danio rerio (Zebrafish)
• Total number of polymer chains: 1
• Total formula weight: 33361.81

Authors

Kuo, Y.C.,Zhang, X. (deposition date: 2018-04-13, release date: 2018-07-18, Last modification date: 2023-10-04)

Primary citation

Kuo, Y.C.,He, X.,Coleman, A.J.,Chen, Y.J.,Dasari, P.,Liou, J.,Biederer, T.,Zhang, X.
Structural analyses of FERM domain-mediated membrane localization of FARP1.
Sci Rep, 8:10477-10477, 2018

PubMed Abstract: FARP1 is a multi-domain protein that is involved in regulating neuronal development through interacting with cell surface proteins such as class A Plexins and SynCAM 1. The N-terminal FERM domain in FARP1 is known to both promote membrane localization and mediate these protein interactions, for which the underlying molecular mechanisms remain unclear. Here we determined the crystal structures of the FERM domain of FARP1 from zebrafish, and those of FARP2 (a close homolog of FARP1) from mouse and zebrafish. These FERM domains adopt the three-leaved clover fold that is typical of all FERM domains. Our structures reveal a positively charged surface patch that is highly conserved in the FERM domain of FARP1 and FARP2. In vitro lipid-binding experiments showed that the FARP1 FERM domain binds specifically to several types of phospholipid, which is dependent on the positively charged surface patch. We further determined through cell-based analyses that this surface patch on the FERM domain underlies the localization of FARP1 to the plasma membrane, and that FERM domain interactions recruit it to postsynaptic sites in neurons.

PubMed: 29992992
DOI: 10.1038/s41598-018-28692-4

Experimental method

X-RAY DIFFRACTION (2.99 Å)