6D2Q

Crystal structure of the FERM domain of zebrafish FARP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-13
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.97926
Spacegroup name	P 21 21 21
Unit cell lengths	56.031, 59.436, 97.462
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.770 - 2.990
R-factor	0.23
Rwork	0.224
R-free	0.28270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6d2k
RMSD bond length	0.002
RMSD bond angle	0.471
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.770
High resolution limit [Å]	2.990
Number of reflections	6910
<I/σ(I)>	12.75
Completeness [%]	99.3
Redundancy	11.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293	0.1 M Bicine pH 9.0, 16 % MPD (v/v)