6D0Y

X-ray Crystal Structure of PGC-1beta C-terminus bound to the CBP80-CBP20 Cap Binding Complex

6D0Y の概要

• エントリーDOI: 10.2210/pdb6d0y/pdb
• 分子名称: Nuclear cap-binding protein subunit 1, Nuclear cap-binding protein subunit 2, Peroxisome proliferator-activated receptor gamma coactivator 1-beta, ... (6 entities in total)
• 機能のキーワード: cap-binding, m7gpppa, transcription, mrna
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 115455.11

構造登録者

Gleghorn, M.L.,Maquat, L.E. (登録日: 2018-04-11, 公開日: 2018-04-25, 最終更新日: 2024-11-13)

主引用文献

Cho, H.,Rambout, X.,Gleghorn, M.L.,Nguyen, P.Q.T.,Phipps, C.R.,Miyoshi, K.,Myers, J.R.,Kataoka, N.,Fasan, R.,Maquat, L.E.
Transcriptional coactivator PGC-1 alpha contains a novel CBP80-binding motif that orchestrates efficient target gene expression.
Genes Dev., 32:555-567, 2018

PubMed Abstract: Although peroxisome proliferator-activated receptor-γ (PPARγ) coactivator 1α (PGC-1α) is a well-established transcriptional coactivator for the metabolic adaptation of mammalian cells to diverse physiological stresses, the molecular mechanism by which it functions is incompletely understood. Here we used in vitro binding assays, X-ray crystallography, and immunoprecipitations of mouse myoblast cell lysates to define a previously unknown cap-binding protein 80 (CBP80)-binding motif (CBM) in the C terminus of PGC-1α. We show that the CBM, which consists of a nine-amino-acid α helix, is critical for the association of PGC-1α with CBP80 at the 5' cap of target transcripts. Results from RNA sequencing demonstrate that the PGC-1α CBM promotes RNA synthesis from promyogenic genes. Our findings reveal a new conduit between DNA-associated and RNA-associated proteins that functions in a cap-binding protein surveillance mechanism, without which efficient differentiation of myoblasts to myotubes fails to occur.

PubMed: 29654059
DOI: 10.1101/gad.309773.117

実験手法

X-RAY DIFFRACTION (2.676 Å)