6D08
Crystal structure of an engineered bump-hole complex of mutant human chromobox homolog 1 (CBX1) with H3K9bn peptide
Summary for 6D08
| Entry DOI | 10.2210/pdb6d08/pdb |
| Descriptor | Chromobox protein homolog 1, Histone H3.1, GLYCEROL, ... (5 entities in total) |
| Functional Keywords | lysine modification, chromodomain, bump-hole, epigenetics, protein binding |
| Biological source | Homo sapiens (Human) More |
| Total number of polymer chains | 4 |
| Total formula weight | 16646.63 |
| Authors | Arora, S.,Horne, W.S.,Islam, K. (deposition date: 2018-04-10, release date: 2019-04-10, Last modification date: 2023-11-15) |
| Primary citation | Arora, S.,Horne, W.S.,Islam, K. Engineering Methyllysine Writers and Readers for Allele-Specific Regulation of Protein-Protein Interactions. J.Am.Chem.Soc., 141:15466-15470, 2019 Cited by PubMed Abstract: Protein-protein interactions mediated by methyllysine are ubiquitous in biological systems. Specific perturbation of such interactions has remained a challenging endeavor. Herein, we describe an allele-specific strategy toward an engineered protein-protein interface orthogonal to the human proteome. We develop a methyltransferase (writer) variant that installs aryllysine moiety on histones that can only be recognized by an engineered chromodomain (reader). We establish biochemical integrity of the engineered interface, provide structural evidence for orthogonality and validate its applicability to identify transcriptional regulators. Our approach provides an unprecedented strategy for specific manipulation of the methyllysine interactome. PubMed: 31518125DOI: 10.1021/jacs.9b05725 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.1 Å) |
Structure validation
Download full validation report
