6CYV

E. coli DHFR ternary complex with NADP and dihydrofolate

Summary for 6CYV

• Entry DOI: 10.2210/pdb6cyv/pdb
• Related: 6CW7 6CXK
• Descriptor: Dihydrofolate reductase, NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, DIHYDROFOLIC ACID, ... (4 entities in total)
• Functional Keywords: dhfr, dihydrofolate reductase, dihydrofolate, nadp, ternary complex, oxidoreductase
• Biological source: Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
• Total number of polymer chains: 1
• Total formula weight: 20035.04

Authors

Cao, H.,Rodrigues, J.,Benach, J.,Frommelt, A.,Morisco, L.,Koss, J.,Shakhnovich, E.,Skolnick, J. (deposition date: 2018-04-06, release date: 2019-01-09, Last modification date: 2023-10-04)

Primary citation

Cao, H.,Gao, M.,Zhou, H.,Skolnick, J.
The crystal structure of a tetrahydrofolate-bound dihydrofolate reductase reveals the origin of slow product release.
Commun Biol, 1:226-226, 2018

PubMed Abstract: Dihydrofolate reductase (DHFR) catalyzes the stereospecific reduction of 7,8-dihydrofolate (FH2) to (6s)-5,6,7,8-tetrahydrofolate (FH4) via hydride transfer from NADPH. The consensus DHFR mechanism involves conformational changes between closed and occluded states occurring during the rate-limiting product release step. Although the Protein Data Bank (PDB) contains over 250 DHFR structures, the FH4 complex structure responsible for rate-limiting product release is unknown. We report to our knowledge the first crystal structure of an . DHFR:FH4 complex at 1.03 Å resolution showing distinct stabilizing interactions absent in FH2 or related (6R)-5,10-dideaza-FH4 complexes. We discover the time course of decay of the co-purified endogenous FH4 during crystal growth, with conversion from FH4 to FH2 occurring in 2-3 days. We also determine another occluded complex structure of DHFR with a slow-onset nanomolar inhibitor that contrasts with the methotrexate complex, suggesting a plausible strategy for designing DHFR antibiotics by targeting FH4 product conformations.

PubMed: 30564747
DOI: 10.1038/s42003-018-0236-y

Experimental method

X-RAY DIFFRACTION (1.3 Å)