6CJK

Anti HIV Fab 10A

Summary for 6CJK

• Entry DOI: 10.2210/pdb6cjk/pdb
• Descriptor: Immunoglobulin Fab light chain, Immunoglobulin Fab heavy chain, ACETATE ION, ... (5 entities in total)
• Functional Keywords: fab, hiv, immune system
• Biological source: Oryctolagus cuniculus (rabbit); More
• Total number of polymer chains: 4
• Total formula weight: 93096.46

Authors

Hangartner, L.,Ward, A.B.,Wilson, I.A.,Oyen, D. (deposition date: 2018-02-26, release date: 2018-08-15, Last modification date: 2024-11-06)

Primary citation

Bianchi, M.,Turner, H.L.,Nogal, B.,Cottrell, C.A.,Oyen, D.,Pauthner, M.,Bastidas, R.,Nedellec, R.,McCoy, L.E.,Wilson, I.A.,Burton, D.R.,Ward, A.B.,Hangartner, L.
Electron-Microscopy-Based Epitope Mapping Defines Specificities of Polyclonal Antibodies Elicited during HIV-1 BG505 Envelope Trimer Immunization.
Immunity, 49:288-300.e8, 2018

PubMed Abstract: Characterizing polyclonal antibody responses via currently available methods is inherently complex and difficult. Mapping epitopes in an immune response is typically incomplete, which creates a barrier to fully understanding the humoral response to antigens and hinders rational vaccine design efforts. Here, we describe a method of characterizing polyclonal responses by using electron microscopy, and we applied this method to the immunization of rabbits with an HIV-1 envelope glycoprotein vaccine candidate, BG505 SOSIP.664. We detected known epitopes within the polyclonal sera and revealed how antibody responses evolved during the prime-boosting strategy to ultimately result in a neutralizing antibody response. We uncovered previously unidentified epitopes, including an epitope proximal to one recognized by human broadly neutralizing antibodies as well as potentially distracting non-neutralizing epitopes. Our method provides an efficient and semiquantitative map of epitopes that are targeted in a polyclonal antibody response and should be of widespread utility in vaccine and infection studies.

PubMed: 30097292
DOI: 10.1016/j.immuni.2018.07.009

Experimental method

X-RAY DIFFRACTION (1.795 Å)