6CJK
Anti HIV Fab 10A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.03315 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.173, 63.558, 72.649 |
| Unit cell angles | 67.99, 87.87, 76.73 |
Refinement procedure
| Resolution | 40.703 - 1.795 |
| R-factor | 0.1656 |
| Rwork | 0.163 |
| R-free | 0.20560 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.073 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.703 | 1.830 |
| High resolution limit [Å] | 1.795 | 1.800 |
| Rpim | 0.048 | |
| Number of reflections | 73928 | |
| <I/σ(I)> | 16.5 | |
| Completeness [%] | 96.3 | |
| Redundancy | 3.2 | |
| CC(1/2) | 0.856 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M sodium citrate pH 5.26, 0.17 M ammonium acetate, 15% glycerol, 19% PEG4000 |
