6C31

Crystal structure of TetR family protein Rv0078 in complex with DNA

Summary for 6C31

• Entry DOI: 10.2210/pdb6c31/pdb
• Related: 5WM9
• Descriptor: TetR family transcriptional regulator, DNA (5'-D(P*AP*CP*AP*AP*GP*CP*AP*GP*AP*CP*TP*GP*CP*CP*GP*GP*TP*AP*AP*C)-3'), DNA (5'-D(*GP*TP*TP*AP*CP*CP*GP*GP*CP*AP*GP*TP*CP*TP*GP*CP*TP*TP*GP*TP*AP*AP*A)-3') (3 entities in total)
• Functional Keywords: rv0078, dna, tetr family, dna binding protein-dna complex, dna binding protein/dna
• Biological source: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv); More
• Total number of polymer chains: 12
• Total formula weight: 216065.66

Authors

Hsu, H.C.,Li, H. (deposition date: 2018-01-09, release date: 2018-07-04, Last modification date: 2023-10-04)

Primary citation

Samanovic, M.I.,Hsu, H.C.,Jones, M.B.,Jones, V.,McNeil, M.R.,Becker, S.H.,Jordan, A.T.,Strnad, M.,Xu, C.,Jackson, M.,Li, H.,Darwin, K.H.
Cytokinin Signaling in Mycobacterium tuberculosis.
MBio, 9:-, 2018

PubMed Abstract: It was recently reported that the human-exclusive pathogen secretes cytokinins, which had only been known as plant hormones. While cytokinins are well-established, adenine-based signaling molecules in plants, they have never been shown to participate in signal transduction in other kingdoms of life. is not known to interact with plants. Therefore, we tested the hypothesis that cytokinins trigger transcriptional changes within this bacterial species. Here, we show cytokinins induced the strong expression of the gene Rv0077c. We found that Rv0077c expression is repressed by a TetR-like transcriptional repressor, Rv0078. Strikingly, cytokinin-induced expression of Rv0077c resulted in a loss of acid-fast staining of While acid-fast staining is thought to be associated with changes in the bacterial cell envelope and virulence, Rv0077c-induced loss of acid-fastness did not affect antibiotic susceptibility or attenuate bacterial growth in mice, consistent with an unaltered mycolic acid profile of Rv0077c-expressing cells. Collectively, these findings show cytokinins signal transcriptional changes that can affect acid-fastness and that cytokinin signaling is no longer limited to the kingdom Plantae. Cytokinins have only previously been known as plant hormones. The discovery that they can be used as signaling molecules outside of plants broadens the repertoire of small molecules that can potentially affect gene expression in all domains of life.

PubMed: 29921668
DOI: 10.1128/mBio.00989-18

Experimental method

X-RAY DIFFRACTION (3 Å)