6C31
Crystal structure of TetR family protein Rv0078 in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-08-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.12713 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.039, 80.040, 186.660 |
| Unit cell angles | 90.00, 90.07, 90.00 |
Refinement procedure
| Resolution | 28.495 - 3.000 |
| R-factor | 0.2065 |
| Rwork | 0.202 |
| R-free | 0.24690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5wm9 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.740 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rpim | 0.087 | 0.474 |
| Number of reflections | 46330 | |
| <I/σ(I)> | 5.5 | |
| Completeness [%] | 94.8 | |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M magnesium formate dihydrate |
