6C24

Cryo-EM structure of PRC2 bound to cofactors AEBP2 and JARID2 in the Extended Active State

6C24 の概要

• エントリーDOI: 10.2210/pdb6c24/pdb
• EMDBエントリー: 7334 7335
• 分子名称: Polycomb protein SUZ12, Protein Jumonji, Histone-lysine N-methyltransferase EZH2, ... (9 entities in total)
• 機能のキーワード: polycomb repressive complex, aebp2, jarid2, histone modification, gene regulation
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 639134.12

構造登録者

Kasinath, V.,Faini, M.,Poepsel, S.,Reif, D.,Feng, A.,Stjepanovic, G.,Aebersold, R.,Nogales, E. (登録日: 2018-01-06, 公開日: 2018-01-24, 最終更新日: 2025-04-09)

主引用文献

Kasinath, V.,Faini, M.,Poepsel, S.,Reif, D.,Feng, X.A.,Stjepanovic, G.,Aebersold, R.,Nogales, E.
Structures of human PRC2 with its cofactors AEBP2 and JARID2.
Science, 359:940-944, 2018

PubMed Abstract: Transcriptionally repressive histone H3 lysine 27 methylation by Polycomb repressive complex 2 (PRC2) is essential for cellular differentiation and development. Here we report cryo-electron microscopy structures of human PRC2 in a basal state and two distinct active states while in complex with its cofactors JARID2 and AEBP2. Both cofactors mimic the binding of histone H3 tails. JARID2, methylated by PRC2, mimics a methylated H3 tail to stimulate PRC2 activity, whereas AEBP2 interacts with the RBAP48 subunit, mimicking an unmodified H3 tail. SUZ12 interacts with all other subunits within the assembly and thus contributes to the stability of the complex. Our analysis defines the complete architecture of a functionally relevant PRC2 and provides a structural framework to understand its regulation by cofactors, histone tails, and RNA.

PubMed: 29348366
DOI: 10.1126/science.aar5700

実験手法

ELECTRON MICROSCOPY (3.5 Å)