6BZ8

Thermus thermophilus 70S containing 16S G347U point mutation and near-cognate ASL Leucine in A site

This is a large structure.

Summary for 6BZ8

Descriptor16S rRNA, 30S ribosomal protein S2, 30S ribosomal protein S3, ... (58 entities in total)
Functional Keywordsprotein biosynthesis, ribosomes, rna, trna, transfer rna, 30s, 50s, 70s, 16s, 23s, ribosomal subunit, bacterial proteins, codon, transfer, translational, ribosome
Biological sourceThermus thermophilus HB8
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Total number of polymer chains112
Total molecular weight4487952.83
Authors
Hoffer, E.D.,Maehigashi, T.,Fagan, C.E.,Dunham, C.M. (deposition date: 2017-12-22, release date: 2018-11-14, Last modification date: 2019-02-20)
Primary citation
Hoffer, E.D.,Maehigashi, T.,Fredrick, K.,Dunham, C.M.
Ribosomal ambiguity (ram) mutations promote the open (off) to closed (on) transition and thereby increase miscoding.
Nucleic Acids Res., 2018
PubMed: 30476222 (PDB entries with the same primary citation)
DOI: 10.1093/nar/gky1178
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (3.74 Å)
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Structure validation

ClashscoreRamachandran outliersSidechain outliersRNA backbone6 0.5% 0.7% 0.54MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 6bz8
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Molmil generated image of 6bz8
rotated about x axis by 90°
Molmil generated image of 6bz8
rotated about y axis by 90°
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