6BZ8
Thermus thermophilus 70S containing 16S G347U point mutation and near-cognate ASL Leucine in A site
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-10-04 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 210.805, 448.832, 618.447 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 134.676 - 3.740 |
R-factor | 0.2054 |
Rwork | 0.204 |
R-free | 0.22460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4v97 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.107 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX (1.12_2829) |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 134.676 | 134.676 | 3.700 |
High resolution limit [Å] | 3.610 | 16.140 | 3.610 |
Rmerge | 0.225 | 0.049 | 1.253 |
Rmeas | 0.235 | 0.052 | 1.352 |
Total number of observations | 7986683 | ||
Number of reflections | 666085 | 7954 | 49062 |
<I/σ(I)> | 9.79 | 40.01 | 1.52 |
Completeness [%] | 100.0 | 99.3 | 99.9 |
Redundancy | 11.99 | 12.664 | 7.036 |
CC(1/2) | 0.991 | 0.989 | 0.530 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 4.7% PEG 20k, 4.7% PEG 550MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC |