6BY5

Two-State 14-mer UUCG Tetraloop calculated from Exact NOEs (State one: Conformers 1-5, State Two: Conformers 6-10)

Summary for 6BY5

• Entry DOI: 10.2210/pdb6by5/pdb
• Related: 6BY4
• NMR Information: BMRB: 30386
• Descriptor: RNA (5'-R(P*GP*GP*CP*AP*CP*UP*UP*CP*GP*GP*UP*GP*CP*C)-3') (1 entity in total)
• Functional Keywords: rna, exact noe, uucg tetraloop, 14-mer hairpin, two-state
• Biological source: synthetic construct
• Total number of polymer chains: 1
• Total formula weight: 4454.68

Authors

Nichols, P.J.,Henen, M.A.,Born, A.,Strotz, D.,Guntert, P.,Vogeli, B. (deposition date: 2017-12-19, release date: 2018-06-13, Last modification date: 2024-05-22)

Primary citation

Nichols, P.J.,Henen, M.A.,Born, A.,Strotz, D.,Guntert, P.,Vogeli, B.
High-resolution small RNA structures from exact nuclear Overhauser enhancement measurements without additional restraints.
Commun Biol, 1:61-61, 2018

PubMed Abstract: RNA not only translates the genetic code into proteins, but also carries out important cellular functions. Understanding such functions requires knowledge of the structure and dynamics at atomic resolution. Almost half of the published RNA structures have been solved by nuclear magnetic resonance (NMR). However, as a result of severe resonance overlap and low proton density, high-resolution RNA structures are rarely obtained from nuclear Overhauser enhancement (NOE) data alone. Instead, additional semi-empirical restraints and labor-intensive techniques are required for structural averages, while there are only a few experimentally derived ensembles representing dynamics. Here we show that our exact NOE (eNOE) based structure determination protocol is able to define a 14-mer UUCG tetraloop structure at high resolution without other restraints. Additionally, we use eNOEs to calculate a two-state structure, which samples its conformational space. The protocol may open an avenue to obtain high-resolution structures of small RNA of unprecedented accuracy with moderate experimental efforts.

PubMed: 30271943
DOI: 10.1038/s42003-018-0067-x

Experimental method

SOLUTION NMR