6BPZ

Structure of the mechanically activated ion channel Piezo1

Summary for 6BPZ

• Entry DOI: 10.2210/pdb6bpz/pdb
• EMDB information: 7128
• Descriptor: Piezo-type mechanosensitive ion channel component 1,Piezo-type mechanosensitive ion channel component 1,mouse Piezo1,Piezo-type mechanosensitive ion channel component 1,Piezo-type mechanosensitive ion channel component 1 (1 entity in total)
• Functional Keywords: mechanosensitive ion channel, membrane protein
• Biological source: Mus musculus (Mouse); More
• Total number of polymer chains: 3
• Total formula weight: 485920.59

Authors

Saotome, K.,Kefauver, J.M.,Patapoutian, A.,Ward, A.B. (deposition date: 2017-11-27, release date: 2017-12-27, Last modification date: 2024-11-06)

Primary citation

Saotome, K.,Murthy, S.E.,Kefauver, J.M.,Whitwam, T.,Patapoutian, A.,Ward, A.B.
Structure of the mechanically activated ion channel Piezo1.
Nature, 554:481-486, 2018

PubMed Abstract: Piezo1 and Piezo2 are mechanically activated ion channels that mediate touch perception, proprioception and vascular development. Piezo proteins are distinct from other ion channels and their structure remains poorly defined, which impedes detailed study of their gating and ion permeation properties. Here we report a high-resolution cryo-electron microscopy structure of the mouse Piezo1 trimer. The detergent-solubilized complex adopts a three-bladed propeller shape with a curved transmembrane region containing at least 26 transmembrane helices per protomer. The flexible propeller blades can adopt distinct conformations, and consist of a series of four-transmembrane helical bundles that we term Piezo repeats. Carboxy-terminal domains line the central ion pore, and the channel is closed by constrictions in the cytosol. A kinked helical beam and anchor domain link the Piezo repeats to the pore, and are poised to control gating allosterically. The structure provides a foundation to dissect further how Piezo channels are regulated by mechanical force.

PubMed: 29261642
DOI: 10.1038/nature25453

Experimental method

ELECTRON MICROSCOPY (3.8 Å)