6BBH

The CRAC channel Orai in an unlatched-closed conformation; K163W loss-of-function mutation

6BBH の概要

• エントリーDOI: 10.2210/pdb6bbh/pdb
• 分子名称: Calcium release-activated calcium channel protein 1 (1 entity in total)
• 機能のキーワード: ion channel, calcium, soce, crac, eukaryotic, open structure, membrane protein
• 由来する生物種: Drosophila melanogaster (Fruit fly)
• タンパク質・核酸の鎖数: 24
• 化学式量合計: 582822.43

構造登録者

Long, S.B.,Hou, X.,Burstein, S. (登録日: 2017-10-18, 公開日: 2018-09-12, 最終更新日: 2024-03-13)

主引用文献

Hou, X.,Burstein, S.R.,Long, S.B.
Structures reveal opening of the store-operated calcium channel Orai.
Elife, 7:-, 2018

PubMed Abstract: The store-operated calcium (Ca) channel Orai governs Ca influx through the plasma membrane of many non-excitable cells in metazoans. The channel opens in response to the depletion of Ca stored in the endoplasmic reticulum (ER). Loss- and gain-of-function mutants of Orai cause disease. Our previous work revealed the structure of Orai with a closed pore. Here, using a gain-of-function mutation that constitutively activates the channel, we present an X-ray structure of Orai in an open conformation. Well-defined electron density maps reveal that the pore is dramatically dilated on its cytosolic side in comparison to the slender closed pore. Cations and anions bind in different regions of the open pore, informing mechanisms for ion permeation and Ca selectivity. Opening of the pore requires the release of cytosolic latches. Together with additional X-ray structures of an unlatched-but-closed conformation, we propose a sequence for store-operated activation.

PubMed: 30160233
DOI: 10.7554/eLife.36758

実験手法

X-RAY DIFFRACTION (6.1 Å)