6B1B

STRUCTURE OF 4-HYDROXYPHENYLACETATE 3-MONOOXYGENASE (HPAB), OXYGENASE COMPONENT FROM ESCHERICHIA COLI MUTANT XS6 (APO Enzyme)

Summary for 6B1B

Related5UM5
Descriptor4-hydroxyphenylacetate 3-monooxygenase, oxygenase subunit, trimethylamine oxide (3 entities in total)
Functional Keywordsprotein engineering: 4-hydroxyphenylacetate 3-monooxygenase, oxygenase component, oxidoreductase active site loop mutant 7.2, oxidoreductase
Biological sourceEscherichia coli (strain B / BL21-DE3)
Total number of polymer chains2
Total molecular weight120137.58
Authors
Zhou, D.,Kandavelu, P.,Wang, B.C.,Yan, Y.,Rose, J.P. (deposition date: 2017-09-18, release date: 2019-05-22, Last modification date: 2019-06-05)
Primary citation
Shen, X.,Zhou, D.,Lin, Y.,Wang, J.,Gao, S.,Kandavelu, P.,Zhang, H.,Zhang, R.,Wang, B.C.,Rose, J.,Yuan, Q.,Yan, Y.
Structural Insights into Catalytic Versatility of the Flavin-dependent Hydroxylase (HpaB) from Escherichia coli.
Sci Rep, 9:7087-7087, 2019
PubMed: 31068633 (PDB entries with the same primary citation)
DOI: 10.1038/s41598-019-43577-w
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.944 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.1665 0.4% 1.9% 2.1%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 6b1b
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More Biological unit images

Molmil generated image of 6b1b
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Molmil generated image of 6b1b
rotated about x axis by 90°
Molmil generated image of 6b1b
rotated about y axis by 90°
(*)In the case of coarse surface representation, the asymmetric unit is shown as red ribbon representation.
Coordinate files for Biological unit (6b1b.pdb1.gz [701.15 KB])