6B1B

STRUCTURE OF 4-HYDROXYPHENYLACETATE 3-MONOOXYGENASE (HPAB), OXYGENASE COMPONENT FROM ESCHERICHIA COLI MUTANT XS6 (APO Enzyme)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	4-hydroxyphenylacetate 3-monooxygenase, oxygenase subunit	polymer	527	59768.3	2	UniProt (A0A140NG21) Pfam (PF11794) Pfam (PF03241) In PDB	Escherichia coli (strain B / BL21-DE3)
2	A, B	trimethylamine oxide	non-polymer		75.1	8	Chemie (TMO)
3		water	water		18.0	1061	Chemie (HOH)

Sequence modifications

A, B: 2 - 520 (UniProt: A0A140NG21)

PDB	External Database	Details
Met -6	-	initiating methionine
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Ser 208	Phe 208	engineered mutation
Asp 211	Ala 211	engineered mutation
Leu 212	Gln 212	engineered mutation
Gly 213	Val 213	engineered mutation
Ser 214	Met 214	engineered mutation
Ser 216	Glu 216	engineered mutation
Asp 217	Asn 217	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	119536.7
Non-Polymers*	Number of molecules	8
	Total formula weight	600.9
All*	Total formula weight	120137.6

*Water molecules are not included.