6APD

Crystal structure of RSV F bound by AM22 and the infant antibody ADI-19425

6APD の概要

• エントリーDOI: 10.2210/pdb6apd/pdb
• 分子名称: Fusion glycoprotein F0,Envelope glycoprotein, AM22 Fab Heavy Chain,IGH@ protein, AM22 Fab Light Chain,Uncharacterized protein, ... (5 entities in total)
• 機能のキーワード: viral glycoprotein, immunoglobulin, infant, complex, immune system
• 由来する生物種: Respiratory syncytial virus; 詳細
• タンパク質・核酸の鎖数: 15
• 化学式量合計: 474123.09

構造登録者

Wrapp, D.,McLellan, J.S. (登録日: 2017-08-17, 公開日: 2018-03-21, 最終更新日: 2024-11-06)

主引用文献

Goodwin, E.,Gilman, M.S.A.,Wrapp, D.,Chen, M.,Ngwuta, J.O.,Moin, S.M.,Bai, P.,Sivasubramanian, A.,Connor, R.I.,Wright, P.F.,Graham, B.S.,McLellan, J.S.,Walker, L.M.
Infants Infected with Respiratory Syncytial Virus Generate Potent Neutralizing Antibodies that Lack Somatic Hypermutation.
Immunity, 48:339-349.e5, 2018

PubMed Abstract: Respiratory syncytial virus (RSV) is a leading cause of infant mortality, and there are currently no licensed vaccines to protect this vulnerable population. A comprehensive understanding of infant antibody responses to natural RSV infection would facilitate vaccine development. Here, we isolated more than 450 RSV fusion glycoprotein (F)-specific antibodies from 7 RSV-infected infants and found that half of the antibodies recognized only two antigenic sites. Antibodies targeting both sites showed convergent sequence features, and structural studies revealed the molecular basis for their recognition of RSV F. A subset of antibodies targeting one of these sites displayed potent neutralizing activity despite lacking somatic mutations, and similar antibodies were detected in RSV-naive B cell repertoires, suggesting that expansion of these B cells in infants may be possible with suitably designed vaccine antigens. Collectively, our results provide fundamental insights into infant antibody responses and a framework for the rational design of age-specific RSV vaccines.

PubMed: 29396163
DOI: 10.1016/j.immuni.2018.01.005

実験手法

X-RAY DIFFRACTION (4.1 Å)