6AJI

Crystal structure of mycolic acid transporter MmpL3 from Mycobacterium smegmatis complexed with Rimonabant

Summary for 6AJI

• Entry DOI: 10.2210/pdb6aji/pdb
• Descriptor: Drug exporters of the RND superfamily-like protein,Endolysin, 5-(4-chlorophenyl)-1-(2,4-dichlorophenyl)-4-methyl-N-(piperidin-1-yl)-1H-pyrazole-3-carboxamide, alpha-D-glucopyranosyl 6-O-dodecyl-alpha-D-glucopyranoside, ... (4 entities in total)
• Functional Keywords: membrane protein, transporter, rnd family, cell wall biosynthesis, drug target, hydrolase
• Biological source: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155); More
• Total number of polymer chains: 1
• Total formula weight: 106383.57

Authors

Zhang, B.,Li, J.,Yang, X.L.,Wu, L.J.,Yang, H.T.,Rao, Z.H. (deposition date: 2018-08-27, release date: 2018-12-26, Last modification date: 2023-11-22)

Primary citation

Zhang, B.,Li, J.,Yang, X.,Wu, L.,Zhang, J.,Yang, Y.,Zhao, Y.,Zhang, L.,Yang, X.,Yang, X.,Cheng, X.,Liu, Z.,Jiang, B.,Jiang, H.,Guddat, L.W.,Yang, H.,Rao, Z.
Crystal Structures of Membrane Transporter MmpL3, an Anti-TB Drug Target.
Cell, 176:636-648.e13, 2019

PubMed Abstract: Despite intensive efforts to discover highly effective treatments to eradicate tuberculosis (TB), it remains as a major threat to global human health. For this reason, new TB drugs directed toward new targets are highly coveted. MmpLs (Mycobacterial membrane proteins Large), which play crucial roles in transporting lipids, polymers and immunomodulators and which also extrude therapeutic drugs, are among the most important therapeutic drug targets to emerge in recent times. Here, crystal structures of mycobacterial MmpL3 alone and in complex with four TB drug candidates, including SQ109 (in Phase 2b-3 clinical trials), are reported. MmpL3 consists of a periplasmic pore domain and a twelve-helix transmembrane domain. Two Asp-Tyr pairs centrally located in this domain appear to be key facilitators of proton-translocation. SQ109, AU1235, ICA38, and rimonabant bind inside the transmembrane region and disrupt these Asp-Tyr pairs. This structural data will greatly advance the development of MmpL3 inhibitors as new TB drugs.

PubMed: 30682372
DOI: 10.1016/j.cell.2019.01.003

Experimental method

X-RAY DIFFRACTION (2.9 Å)