6AHD

The Cryo-EM Structure of Human Pre-catalytic Spliceosome (B complex) at 3.8 angstrom resolution

これはPDB形式変換不可エントリーです。

6AHD の概要

• エントリーDOI: 10.2210/pdb6ahd/pdb
• EMDBエントリー: 9624
• 分子名称: Pre-mRNA-processing-splicing factor 8, U4/U6 small nuclear ribonucleoprotein Prp31, U4/U6.U5 tri-snRNP-associated protein 1, ... (52 entities in total)
• 機能のキーワード: spliceosome, splicing
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 63
• 化学式量合計: 2671164.68

構造登録者

Zhan, X.,Yan, C.,Zhang, X.,Shi, Y. (登録日: 2018-08-17, 公開日: 2018-11-14, 最終更新日: 2024-11-06)

主引用文献

Zhan, X.,Yan, C.,Zhang, X.,Lei, J.,Shi, Y.
Structures of the human pre-catalytic spliceosome and its precursor spliceosome.
Cell Res., 28:1129-1140, 2018

PubMed Abstract: The pre-catalytic spliceosome (B complex) is preceded by its precursor spliceosome (pre-B complex) and followed by the activated spliceosome (B complex). The pre-B-to-B and B-to-B transitions are driven by the ATPase/helicases Prp28 and Brr2, respectively. In this study, we report the cryo-electron microscopy structures of the human pre-B complex and the human B complex at an average resolution of 5.7 and 3.8 Å, respectively. In the pre-B complex, U1 and U2 small nuclear ribonucleoproteins (snRNPs) associate with two edges of the tetrahedron-shaped U4/U6.U5 tri-snRNP. The pre-mRNA is yet to be recognized by U5 or U6 small nuclear RNA (snRNA), and loop I of U5 snRNA remains unengaged. In the B complex, U1 snRNP and Prp28 are dissociated, the 5'-exon is anchored to loop I of U5 snRNA, and the 5'-splice site is recognized by U6 snRNA through duplex formation. In sharp contrast to S. cerevisiae, most components of U2 snRNP and tri-snRNP, exemplified by Brr2, undergo pronounced rearrangements in the human pre-B-to-B transition. Structural analysis reveals mechanistic insights into the assembly and activation of the human spliceosome.

PubMed: 30315277
DOI: 10.1038/s41422-018-0094-7

実験手法

ELECTRON MICROSCOPY (3.8 Å)