6AEB

Crystal structure of xCas9 in complex with sgRNA and target DNA (AAG PAM)

Summary for 6AEB

• Entry DOI: 10.2210/pdb6aeb/pdb
• Descriptor: RNA (95-MER), DNA (25-MER), DNA (5'-D(*AP*AP*AP*AP*AP*GP*TP*AP*TP*TP*G)-3'), ... (5 entities in total)
• Functional Keywords: enzyme, dna binding protein-rna complex, dna binding protein/rna
• Biological source: Streptococcus pyogenes; More
• Total number of polymer chains: 8
• Total formula weight: 405543.75

Authors

Guo, M.,Ren, K.,Zhu, Y.,Huang, Z. (deposition date: 2018-08-04, release date: 2019-03-27, Last modification date: 2023-11-22)

• Primary citation: Guo, M.,Ren, K.,Zhu, Y.,Tang, Z.,Wang, Y.,Zhang, B.,Huang, Z.; Structural insights into a high fidelity variant of SpCas9.; Cell Res., 29:183-192, 2019; PubMed: 30664728; DOI: 10.1038/s41422-018-0131-6

Experimental method

X-RAY DIFFRACTION (3.004 Å)