6AEB
Crystal structure of xCas9 in complex with sgRNA and target DNA (AAG PAM)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E (A, E) | RNA (95-MER) | polymer | 100 | 32269.2 | 2 | Streptococcus pyogenes | ||
| 2 | B, F (C, G) | DNA (25-MER) | polymer | 28 | 8558.6 | 2 | Streptococcus pyogenes | ||
| 3 | C, G (D, H) | DNA (5'-D(*AP*AP*AP*AP*AP*GP*TP*AP*TP*TP*G)-3') | polymer | 11 | 3405.3 | 2 | Streptococcus pyogenes | ||
| 4 | D, H (B, F) | DNA Nuclease | polymer | 1368 | 158538.8 | 2 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) | Streptococcus pyogenes | |
| 5 | I (B) | water | water | 18.0 | 1 | Chemie (HOH) |
Sequence modifications
B, F: 1 - 1368 (UniProt: Q99ZW2)
| PDB | External Database | Details |
|---|---|---|
| Ala 10 | Asp 10 | engineered mutation |
| Thr 262 | Ala 262 | engineered mutation |
| Leu 324 | Arg 324 | engineered mutation |
| Ile 409 | Ser 409 | engineered mutation |
| Lys 480 | Glu 480 | engineered mutation |
| Asp 543 | Glu 543 | engineered mutation |
| Ile 694 | Met 694 | engineered mutation |
| Ala 840 | His 840 | engineered mutation |
| Val 1219 | Glu 1219 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 405543.8 | |
| All* | Total formula weight | 405543.8 |
