6S21

Metabolism of multiple glycosaminoglycans by bacteroides thetaiotaomicron is orchestrated by a versatile core genetic locus (BT33494S-sulf)

Summary for 6S21

• Entry DOI: 10.2210/pdb6s21/pdb
• Descriptor: Endo-4-O-sulfatase, 4-deoxy-alpha-L-threo-hex-4-enopyranuronic acid-(1-3)-2-acetamido-2-deoxy-4-O-sulfo-beta-D-galactopyranose-(1-4)-beta-D-glucopyranuronic acid, CALCIUM ION, ... (4 entities in total)
• Functional Keywords: gut microbiota, glycosaminoglycan, glycobiology, cazymes, hydrolase
• Biological source: Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
• Total number of polymer chains: 3
• Total formula weight: 177978.39

Authors

Ndeh, D.,Basle, A.,Strahl, H.,Henrissat, B.,Terrapon, N.,Cartmell, A. (deposition date: 2019-06-19, release date: 2020-02-05, Last modification date: 2024-05-15)

Primary citation

Ndeh, D.,Basle, A.,Strahl, H.,Yates, E.A.,McClurgg, U.L.,Henrissat, B.,Terrapon, N.,Cartmell, A.
Metabolism of multiple glycosaminoglycans by Bacteroides thetaiotaomicron is orchestrated by a versatile core genetic locus.
Nat Commun, 11:646-646, 2020

PubMed Abstract: The human gut microbiota (HGM), which is critical to human health, utilises complex glycans as its major carbon source. Glycosaminoglycans represent an important, high priority, nutrient source for the HGM. Pathways for the metabolism of various glycosaminoglycan substrates remain ill-defined. Here we perform a biochemical, genetic and structural dissection of the genetic loci that orchestrates glycosaminoglycan metabolism in the organism Bacteroides thetaiotaomicron. Here, we report: the discovery of two previously unknown surface glycan binding proteins which facilitate glycosaminoglycan import into the periplasm; distinct kinetic and genetic specificities of various periplasmic lyases which dictate glycosaminoglycan metabolic pathways; understanding of endo sulfatase activity questioning the paradigm of how the 'sulfation problem' is handled by the HGM; and 3D crystal structures of the polysaccharide utilisation loci encoded sulfatases. Together with comparative genomic studies, our study fills major gaps in our knowledge of glycosaminoglycan metabolism by the HGM.

PubMed: 32005816
DOI: 10.1038/s41467-020-14509-4

Experimental method

X-RAY DIFFRACTION (2.8 Å)