6QFA
CryoEM structure of a beta3K279T GABA(A)R homomer in complex with histamine and megabody Mb25
Summary for 6QFA
Entry DOI | 10.2210/pdb6qfa/pdb |
EMDB information | 4542 |
Descriptor | Gamma-aminobutyric acid receptor subunit beta-3,Gamma-aminobutyric acid receptor subunit beta-3, Outer membrane protein,Outer membrane protein,Outer membrane protein,Outer membrane protein,Uncharacterized protein,Uncharacterized protein,Mb-c7HopQ-Nb25, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total) |
Functional Keywords | megabody, membrane protein, protein engineering, cryo-em |
Biological source | Homo sapiens (Human) More |
Total number of polymer chains | 10 |
Total formula weight | 485236.80 |
Authors | Uchanski, T.,Masiulis, S.,Fischer, B.,Kalichuk, V.,Wohlkoening, A.,Zoegg, T.,Remaut, H.,Vranken, W.,Aricescu, A.R.,Pardon, E.,Steyaert, J. (deposition date: 2019-01-09, release date: 2021-08-04) |
Primary citation | Uchanski, T.,Masiulis, S.,Fischer, B.,Kalichuk, V.,Lopez-Sanchez, U.,Zarkadas, E.,Weckener, M.,Sente, A.,Ward, P.,Wohlkonig, A.,Zogg, T.,Remaut, H.,Naismith, J.H.,Nury, H.,Vranken, W.,Aricescu, A.R.,Pardon, E.,Steyaert, J. Megabodies expand the nanobody toolkit for protein structure determination by single-particle cryo-EM. Nat.Methods, 18:60-68, 2021 Cited by PubMed: 33408403DOI: 10.1038/s41592-020-01001-6 PDB entries with the same primary citation |
Experimental method | ELECTRON MICROSCOPY (2.49 Å) |
Structure validation
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