6EA8

Structure of VACV poxin in pre-reactive state with nonhydrolyzable 2'3' cGAMP

Summary for 6EA8

• Entry DOI: 10.2210/pdb6ea8/pdb
• Descriptor: Protein B2, (2S,5R,7R,8R,10R,12aR,14R,15R,15aS,16R)-7-(2-amino-6-oxo-3,6-dihydro-9H-purin-9-yl)-14-(6-amino-9H-purin-9-yl)-15,16-dihydroxy-2,10-disulfanyloctahydro-2H,10H,12H-5,8-methano-2lambda~5~,10lambda~5~-furo[3,2-l][1,3,6,9,11,2,10]pentaoxadiphosphacyclotetradecine-2,10-dione, O-[(1R,2R,3R)-5-{[(S)-{[(2R,3R,4R,5R)-2-(2-amino-6-oxo-3,6-dihydro-9H-purin-9-yl)-4-hydroxy-5-(hydroxymethyl)tetrahydro furan-3-yl]oxy}(sulfanyl)phosphoryl]oxy}-1-(6-amino-9H-purin-9-yl)-1,2-dihydroxypentan-3-yl] dihydrogen (R)-phosphorothioate, ... (4 entities in total)
• Functional Keywords: vaccina virus, nuclease, cgas, cgamp, poxvirus, innate immunity, hydrolase
• Biological source: Vaccinia virus WR (VACV)
• Total number of polymer chains: 10
• Total formula weight: 249571.93

Authors

Eaglesham, J.B.,Kranzusch, P.J. (deposition date: 2018-08-02, release date: 2019-02-06, Last modification date: 2024-03-13)

• Primary citation: Eaglesham, J.B.,Pan, Y.,Kupper, T.S.,Kranzusch, P.J.; Viral and metazoan poxins are cGAMP-specific nucleases that restrict cGAS-STING signalling.; Nature, 566:259-263, 2019; PubMed: 30728498; DOI: 10.1038/s41586-019-0928-6

Experimental method

X-RAY DIFFRACTION (2.6 Å)