6EA8

Structure of VACV poxin in pre-reactive state with nonhydrolyzable 2'3' cGAMP

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004518	molecular_function	nuclease activity
A	0016787	molecular_function	hydrolase activity
A	0030430	cellular_component	host cell cytoplasm
A	0052170	biological_process	symbiont-mediated suppression of host innate immune response
A	0061507	molecular_function	2',3'-cyclic GMP-AMP binding
B	0004518	molecular_function	nuclease activity
B	0016787	molecular_function	hydrolase activity
B	0030430	cellular_component	host cell cytoplasm
B	0052170	biological_process	symbiont-mediated suppression of host innate immune response
B	0061507	molecular_function	2',3'-cyclic GMP-AMP binding
C	0004518	molecular_function	nuclease activity
C	0016787	molecular_function	hydrolase activity
C	0030430	cellular_component	host cell cytoplasm
C	0052170	biological_process	symbiont-mediated suppression of host innate immune response
C	0061507	molecular_function	2',3'-cyclic GMP-AMP binding
D	0004518	molecular_function	nuclease activity
D	0016787	molecular_function	hydrolase activity
D	0030430	cellular_component	host cell cytoplasm
D	0052170	biological_process	symbiont-mediated suppression of host innate immune response
D	0061507	molecular_function	2',3'-cyclic GMP-AMP binding
E	0004518	molecular_function	nuclease activity
E	0016787	molecular_function	hydrolase activity
E	0030430	cellular_component	host cell cytoplasm
E	0052170	biological_process	symbiont-mediated suppression of host innate immune response
E	0061507	molecular_function	2',3'-cyclic GMP-AMP binding
F	0004518	molecular_function	nuclease activity
F	0016787	molecular_function	hydrolase activity
F	0030430	cellular_component	host cell cytoplasm
F	0052170	biological_process	symbiont-mediated suppression of host innate immune response
F	0061507	molecular_function	2',3'-cyclic GMP-AMP binding
G	0004518	molecular_function	nuclease activity
G	0016787	molecular_function	hydrolase activity
G	0030430	cellular_component	host cell cytoplasm
G	0052170	biological_process	symbiont-mediated suppression of host innate immune response
G	0061507	molecular_function	2',3'-cyclic GMP-AMP binding
H	0004518	molecular_function	nuclease activity
H	0016787	molecular_function	hydrolase activity
H	0030430	cellular_component	host cell cytoplasm
H	0052170	biological_process	symbiont-mediated suppression of host innate immune response
H	0061507	molecular_function	2',3'-cyclic GMP-AMP binding
I	0004518	molecular_function	nuclease activity
I	0016787	molecular_function	hydrolase activity
I	0030430	cellular_component	host cell cytoplasm
I	0052170	biological_process	symbiont-mediated suppression of host innate immune response
I	0061507	molecular_function	2',3'-cyclic GMP-AMP binding
J	0004518	molecular_function	nuclease activity
J	0016787	molecular_function	hydrolase activity
J	0030430	cellular_component	host cell cytoplasm
J	0052170	biological_process	symbiont-mediated suppression of host innate immune response
J	0061507	molecular_function	2',3'-cyclic GMP-AMP binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	16
Details	binding site for residue J2A A 301

Chain	Residue
A	ALA129
A	LYS186
A	HOH407
B	HIS17
B	ALA18
B	PHE19
B	ARG60
B	HOH321
A	VAL130
A	TYR138
A	LYS142
A	ALA145
A	ASN149
A	GLN169
A	ARG182
A	ARG184

---

site_id	AC2
Number of Residues	14
Details	binding site for residue J2A F 301

Chain	Residue
E	ALA129
E	VAL130
E	TYR138
E	LYS142
E	ASN149
E	GLN169
E	ARG182
E	ARG184
E	LYS186
F	GLU14
F	HIS17
F	ALA18
F	ARG60
F	ILE105

---

site_id	AC3
Number of Residues	17
Details	binding site for residue J2B H 301

Chain	Residue
G	ALA129
G	VAL130
G	TYR138
G	LYS142
G	ALA145
G	ASN149
G	ILE167
G	GLN169
G	ARG182
G	ARG184
G	LYS186
H	LEU16
H	HIS17
H	ALA18
H	PHE19
H	ARG60
H	ILE105

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	10
Details	Active site: {"description":"Proton donor","evidences":[{"source":"HAMAP-Rule","id":"MF_04143","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"30728498","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	10
Details	Active site: {"description":"Shared with catalytic histidine of dimeric partner","evidences":[{"source":"HAMAP-Rule","id":"MF_04143","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"30728498","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	10
Details	Active site: {"description":"Proton acceptor; shared with catalytic histidine of dimeric partner","evidences":[{"source":"HAMAP-Rule","id":"MF_04143","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"30728498","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	70
Details	Site: {"description":"Substrate binding","evidences":[{"source":"HAMAP-Rule","id":"MF_04143","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"30728498","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---