5ZND

8-mer nanotube derived from 24-mer rHuHF nanocage

Summary for 5ZND

• Entry DOI: 10.2210/pdb5znd/pdb
• Descriptor: Ferritin heavy chain (2 entities in total)
• Functional Keywords: ferritin, rhuhf, protein redesign, nanotube, metal binding protein
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 1
• Total formula weight: 15762.58

Authors

Wang, W.M.,Wang, L.L.,Zang, J.C.,Chen, H.,Zhao, G.H.,Wang, H.F. (deposition date: 2018-04-09, release date: 2018-11-07, Last modification date: 2023-11-22)

Primary citation

Wang, W.,Wang, L.,Chen, H.,Zang, J.,Zhao, X.,Zhao, G.,Wang, H.
Selective Elimination of the Key Subunit Interfaces Facilitates Conversion of Native 24-mer Protein Nanocage into 8-mer Nanorings.
J. Am. Chem. Soc., 140:14078-14081, 2018

PubMed Abstract: Living systems utilize proteins as building blocks to construct a large variety of self-assembled nanoscale architectures. Yet, creating protein-based assemblies with specific geometries in the laboratory remains challenging. Here, we present a new approach that completely eliminates one natural intersubunit interface of multisubunit protein architecture with high symmetry, resulting in reassembly of the protein architecture into one with lower symmetry. We have applied this approach to the conversion of the 24-mer cage-like ferritin into non-native 8-mer protein nanorings in solution. In the crystal structure, such newly formed nanorings connect with each other through hydrogen bonding in a repeating head-to-tail pattern to form nanotubes, and adjacent nanotubes are staggered relative to one another to create three-dimensional porous protein assemblies. The above strategy allows the study of conversion between protein architectures with different geometries by adjusting the interactions at the intersubunit interfaces, and the fabrication of novel bio-nanomaterials with different geometries.

PubMed: 30336004
DOI: 10.1021/jacs.8b09760

Experimental method

X-RAY DIFFRACTION (3 Å)