5ZBR
Crystal Structure of Kinesin-3 KIF13B motor domain in AMPPNP form
Summary for 5ZBR
Entry DOI | 10.2210/pdb5zbr/pdb |
Descriptor | Kinesin family member 13B, PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, MAGNESIUM ION, ... (5 entities in total) |
Functional Keywords | kinesin, atpase, transport protein |
Biological source | Rattus norvegicus (Rat) |
Total number of polymer chains | 3 |
Total formula weight | 126652.82 |
Authors | |
Primary citation | Ren, J.,Zhang, Y.,Wang, S.,Huo, L.,Lou, J.,Feng, W. Structural Delineation of the Neck Linker of Kinesin-3 for Processive Movement. J. Mol. Biol., 430:2030-2041, 2018 Cited by PubMed Abstract: Processive kinesin motors contain a neck linker (NL) that mediates the chemo-mechanical coupling and controls the directionality and processivity. However, kinesin-3 NL remains poorly determined due to the lack of the structural information of the junction with the following neck coil (NC). Here, we determined the structure of the motor domain (MD)-NL-NC tandem of KIF13B that defines the junction between NL and NC and delineates kinesin-3 NL. Unexpectedly, the length of kinesin-3 NL is much shorter than the previously predicted one. In the MD-NL-NC structure, NL docks onto the MD with a conventional mode but the interaction between NL and the MD is relatively weak due to the shorter N-terminal cover strand of the MD. The optimal short NL and its weak interaction with the MD would generate the tight inter-head strain and facilitate the NL undocking, which may contribute to the fast and superprocessive motility of kinesin-3. PubMed: 29752968DOI: 10.1016/j.jmb.2018.05.010 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2 Å) |
Structure validation
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