5ZBR
Crystal Structure of Kinesin-3 KIF13B motor domain in AMPPNP form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-07-12 |
| Detector | ADSC QUANTUM 1 |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 32 |
| Unit cell lengths | 110.303, 110.303, 95.124 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.105 - 2.000 |
| R-factor | 0.1902 |
| Rwork | 0.189 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gbj |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.935 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.040 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.072 | 0.815 |
| Number of reflections | 85440 | 8768 |
| <I/σ(I)> | 23.8 | 3.2 |
| Completeness [%] | 97.7 | 100 |
| Redundancy | 8 | |
| CC(1/2) | 1.000 | 0.810 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8.1 | 298 | 0.2M sodium citrate dihydrate, 0.1M Bis-Tris propone, 17% w/v PEG 3350 |
