5Z58
Cryo-EM structure of a human activated spliceosome (early Bact) at 4.9 angstrom.
Summary for 5Z58
| Entry DOI | 10.2210/pdb5z58/pdb |
| Related | 5Z56 5Z57 |
| EMDB information | 6889 6890 6891 |
| Descriptor | Pre-mRNA-processing-splicing factor 8, Small nuclear ribonucleoprotein F, Small nuclear ribonucleoprotein E, ... (43 entities in total) |
| Functional Keywords | spliceosome, cryo-em structure, activated spliceosome, early bact complex, pre-mrna splicing, splicing |
| Biological source | Homo sapiens (Human) More |
| Total number of polymer chains | 46 |
| Total formula weight | 2602010.46 |
| Authors | |
| Primary citation | Zhang, X.,Yan, C.,Zhan, X.,Li, L.,Lei, J.,Shi, Y. Structure of the human activated spliceosome in three conformational states. Cell Res., 28:307-322, 2018 Cited by PubMed Abstract: During each cycle of pre-mRNA splicing, the pre-catalytic spliceosome (B complex) is converted into the activated spliceosome (B complex), which has a well-formed active site but cannot proceed to the branching reaction. Here, we present the cryo-EM structure of the human B complex in three distinct conformational states. The EM map allows atomic modeling of nearly all protein components of the U2 small nuclear ribonucleoprotein (snRNP), including three of the SF3a complex and seven of the SF3b complex. The structure of the human B complex contains 52 proteins, U2, U5, and U6 small nuclear RNA (snRNA), and a pre-mRNA. Three distinct conformations have been captured, representing the early, mature, and late states of the human B complex. These complexes differ in the orientation of the Switch loop of Prp8, the splicing factors RNF113A and NY-CO-10, and most components of the NineTeen complex (NTC) and the NTC-related complex. Analysis of these three complexes and comparison with the B and C complexes reveal an ordered flux of components in the B-to-B and the B-to-B transitions, which ultimately prime the active site for the branching reaction. PubMed: 29360106DOI: 10.1038/cr.2018.14 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (4.9 Å) |
Structure validation
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