5YC4

Crystal structure of AL3 PHD finger bound to H3K4me3

Summary for 5YC4

• Entry DOI: 10.2210/pdb5yc4/pdb
• Descriptor: PHD finger protein ALFIN-LIKE 3, Histone H3K4me3, ZINC ION, ... (4 entities in total)
• Functional Keywords: phd finger, transcription
• Biological source: Arabidopsis thaliana (Mouse-ear cress); More
• Total number of polymer chains: 2
• Total formula weight: 7764.52

Authors

Zhao, S.,Zhang, B.,Li, H. (deposition date: 2017-09-06, release date: 2018-01-24, Last modification date: 2023-11-22)

Primary citation

Zhao, S.,Zhang, B.,Yang, M.,Zhu, J.,Li, H.
Systematic Profiling of Histone Readers in Arabidopsis thaliana.
Cell Rep, 22:1090-1102, 2018

PubMed Abstract: Histone post-translational modifications (PTMs) and their recognition by histone readers exert crucial functions in eukaryotes. Despite extensive studies, conservation and diversity of histone PTM regulation between animals and plants remain less explored because of a lack of systematic knowledge of histone readers in plants. Based on a high-throughput surface plasmon resonance imaging (SPRi) platform, we report the lab-on-chip profiling of interactions between 204 putative reader domains and 11 types of histone peptides in Arabidopsis thaliana. Eleven reader hits were then chosen for histone combinatorial readout pattern profiling. Systematic analysis of histone PTM recognition in Arabidopsis thaliana reveals that plant and human histone readers share conservation in domain types and recognition mechanisms. The differences in particular histone mark recognition by transcription regulator EML1 and DNA damage repair factor MSH6 indicate plant-specific histone PTMs function in Arabidopsis thaliana acquired during evolution.

PubMed: 29386129
DOI: 10.1016/j.celrep.2017.12.099

Experimental method

X-RAY DIFFRACTION (2.697 Å)