5Y1I
The crystal structure of GfsF
Summary for 5Y1I
| Entry DOI | 10.2210/pdb5y1i/pdb |
| Descriptor | Cytochrome P450, PROTOPORPHYRIN IX CONTAINING FE, DI(HYDROXYETHYL)ETHER, ... (5 entities in total) |
| Functional Keywords | monooxygenase, heme, oxidoreductase |
| Biological source | Streptomyces graminofaciens |
| Total number of polymer chains | 2 |
| Total formula weight | 90496.74 |
| Authors | Miyanaga, A.,Kudo, F.,Eguchi, T. (deposition date: 2017-07-20, release date: 2017-09-13, Last modification date: 2023-11-22) |
| Primary citation | Miyanaga, A.,Takayanagi, R.,Furuya, T.,Kawamata, A.,Itagaki, T.,Iwabuchi, Y.,Kanoh, N.,Kudo, F.,Eguchi, T. Substrate Recognition by a Dual-Function P450 Monooxygenase GfsF Involved in FD-891 Biosynthesis Chembiochem, 18:2179-2187, 2017 Cited by PubMed Abstract: GfsF is a multifunctional P450 monooxygenase that catalyzes epoxidation and subsequent hydroxylation in the biosynthesis of macrolide polyketide FD-891. Here, we describe the biochemical and structural analysis of GfsF. To obtain the structural basis of a dual-function reaction, we determined the crystal structure of ligand-free GfsF, which revealed GfsF to have a predominantly hydrophobic substrate binding pocket. The docking models, in conjunction with the results of the enzymatic assay with substrate analogues and site-directed mutagenesis suggested two distinct substrate binding modes for epoxidation and hydroxylation reactions, which explained how GfsF regulates the order of two oxidative reactions. These findings provide new insights into the reaction mechanism of multifunctional P450 monooxygenases. PubMed: 28869713DOI: 10.1002/cbic.201700429 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2 Å) |
Structure validation
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