5Y1I
The crystal structure of GfsF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-31 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.080, 102.870, 114.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.490 - 2.000 |
| R-factor | 0.19601 |
| Rwork | 0.194 |
| R-free | 0.23595 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z36 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.819 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.190 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.109 | 0.578 |
| Number of reflections | 50746 | 6781 |
| <I/σ(I)> | 8 | 2.2 |
| Completeness [%] | 94.2 | 87.7 |
| Redundancy | 4.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 299 | 0.2 M KCl, 0.1 M Tris-HCl (pH 8.5) and 27.5% PEG3350 |
